The effect of perinatal treatment with 5HT agonists on monoamine transporters` gene expression in adult rats

Kveštak, Daria (2012) The effect of perinatal treatment with 5HT agonists on monoamine transporters` gene expression in adult rats. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Serotonin (5HT) is a biologically active amine present in mammals in the brain and the peripheral tissues. In the brain, it regulates the development of 5HT neurons and target tissues during neurogenesis, while later it assumes the function of neurotransmitter. Intracellular and extracellular concentration of 5HT is controlled by two types of transporters. Serotonin transporter (5HTt) in brain is responsible for the transport of serotonin from the synaptic cleft into the presynaptic neurons, while vesicular monoamine transporter 2 (VMAT2) is responsible for the transport of monoamines from the cytoplasm of presynaptic neuron into the synaptic vesicles. In this study I examined the impact of the perinatal treatment with 5- hydroxytryptophan or tranylcypromine on the expression of genes for 5HTt and VMAT2 in adult rats. Treatment with 5-hydroxytryptophan induced a significant increase in VMAT2 gene expression in raphe nuclei. Treatment with tranylcypromine induced a significant increase in VMAT2 gene expression in raphe nuclei and significant increase in 5HTt gene expression in frontal cortex. The results suggest that treatment with serotonin agonists have induced long-lasting compensatory changes in the expression of genes involved in central serotonin homeostasis.

Item Type: Thesis (Diploma thesis)
Keywords: 5HT, 5HTt, VMAT2, 5-hydroxytryptophan, tranylcipromine, gene expression
Supervisor: Hranilović, Dubravka
Co-supervisor: Blažević, Sofia
Date: 2012
Number of Pages: 55
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 25 Feb 2014 13:01
Last Modified: 19 Sep 2014 11:12
URI: http://digre.pmf.unizg.hr/id/eprint/498

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