Notch and Ikaros in hematopoietic cell differentiation

Konjević, Matea (2012) Notch and Ikaros in hematopoietic cell differentiation. Diploma thesis, Faculty of Science > Department of Biology.

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Hematopoiesis is the process of formation of new blood cells. Transcription factors from the Ikaros family and Notch signaling pathway regulate important checkpoints during hematopoiesis, which will decide in which direction the cells differentiate. In this work was confirmed and measured expression of transcription factors from the Ikaros family: Ikaros, Helios and Aiolos and Notch signaling pathway: Notch1 and Hes1 in NB4 cells before and after differentiation induced with ATRA. Protocol for the method of multiplex polymerase chain reaction (multiplex PCR) was successfully established, adapted and implemented. Six segments of DNA was simultaneously amplified in a sample of 2500 cells. For multiplex PCR, primers were selected and tested for specificity and to avoid secondary structures. Semi-quantitative RT-PCR was used to detect possible changes in the levels of mRNA in cells before and after differentiation. The reduction in expression of genes Aiolos and Hes1 suggests their possible role in differentiation and development of immature myeloid cells.

Item Type: Thesis (Diploma thesis)
Keywords: transcription factors, multiplex polymerase chain reaction, semi-quantitative RT-PCR, Helios, Aiolos, Hes1
Supervisor: Antica, Mariastefania
Co-supervisor: Matulić, Maja
Date: 2012
Number of Pages: 44
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 03 Mar 2014 13:03
Last Modified: 19 Sep 2014 10:47

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