Effects of Thymoglobuline on the hypoxia-reoxigenation injury in HEK-293 cells inculture

Jurenec, Franjo (2016) Effects of Thymoglobuline on the hypoxia-reoxigenation injury in HEK-293 cells inculture. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Antithymocyte globulines are polyclonal T cell-depleting immunoglobulins used in induction of immunosuppression in kidney transplant recipients. Thymoglobuline, is purified rabbit IgG, obtained by immunization of rabbits with fetal human thymi, which depletes T lymphocytes by complement-dependent lysis and apoptosis. To determine possible direct effects of Thymoglobuline on the cells, we used Human Embryonic Kidney cell line (HEK-293) in culture. We measured membrane potential of the cells using slow whole patch clamp technique. Depolarizations of HEK-293 cells caused by Thymoglobuline were concentration- and membrane potential- dependent, showing direct effect of Thymoglobuline on the HEK-293 cells. The effects of Thymoglobuline in hypoxia/reoxigenation were detected by calculating cell death and determining the cell migration using scratch test. Thymoglobuline prevented the cell death induced by hypoxia and reoxigenation conditions. This is first study showing direct effect of thymoglobulin on HEK-293 cells. The results of this study encourage the research on epithelial cells in transplanted kidneys.

Item Type: Thesis (Doctoral thesis)
Keywords: thymoglobulin, patch-clamp, scratch test, kidney, immunocytochemistry
Supervisor: Knotek, Mladen
Co-supervisor: Sinđić, Aleksandra
Date: 2016
Number of Pages: 101
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 28 Sep 2016 10:54
Last Modified: 28 Sep 2016 10:54
URI: http://digre.pmf.unizg.hr/id/eprint/5120

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