Human papillomavirus (HPV) genotyping in cervical byopsies with abnormal histology

Cindrić, Anita (2012) Human papillomavirus (HPV) genotyping in cervical byopsies with abnormal histology. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Infection with human papillomavirus (HPV) is the main cause of cervical cancer. The literature describes 15 high-risk types of HPV that are associated with progression of premalignant cervical changes, cervical intraepithelial neoplasia (CIN), into cervical cancer. The aim of this study was to examine biopsies of the cervix with a histological diagnosis CIN 1, CIN 2, CIN 3 and cervical cancer, for the presence of HPV DNA using the polymerase chain reaction (PCR), as well as genotype the most common HPV types and determine their frequency in different cervical entities. We analyzed a total of 58 samples, of which 8, 27, 10 and 13 samples had the diagnosis CIN 1, CIN 2, CIN 3 and cancer, respectively. The results of this study indicate that HPV is most commonly present in cervical cancer (92.3%), then in samples with the diagnosis CIN 3 (70.0%), CIN 2 (66.7%) and least in samples with the diagnosis CIN 1 (62.6%). The most commonly typed high-risk HPV was type 16 (27.6%), followed by type 18 (6.9%), whereas the undetermined HPV type was found in 32.8% of cases. Low-risk HPV 6/11 appears in only 1.7% of cases and exclusively in CIN 2 samples.

Item Type: Thesis (Diploma thesis)
Keywords: cervical carcinoma, cervical intraepithelial neoplasia
Supervisor: Grce, Magdalena
Date: 2012
Number of Pages: 45
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 03 Mar 2014 13:31
Last Modified: 19 Sep 2014 09:34
URI: http://digre.pmf.unizg.hr/id/eprint/514

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