The effects of prolonged exposure of recombinant GABAA receptors in cell culture to alcohol and gabapentin

Malešević, Marina (2012) The effects of prolonged exposure of recombinant GABAA receptors in cell culture to alcohol and gabapentin. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Prolonged exposure of GABAA receptors to alcohol triggers changes associated with the development of alcoholism. Gabapentin shows positive effects in alcoholism treatment. The mechanisms of action of both, gabapentin and alcohol, are not clear. Cytotoxic effect of prolonged exposure of HEK 293 cells, expressing α1β2γ2s GABAA receptors, to 100 mM alcohol was reduced by alcohol withdrawal and 1 μM gabapentin treatment. Chronic alcohol treatment induced an increase in the number of benzodiazepine binding sites and allosteric uncoupling, which were counteracted by alcohol withdrawal. Gabapentin did not affect the number of benzodiazepine binding sites, but restored their normal functional interactions. The results suggest that prolonged alcohol treatment induces similar changes of GABAA receptors as prolonged exposure to benzodiazepines, which also produce dependence. Results support the hypothesis of involvement of GABAA receptors in the actions of gabapentin. Further studies should investigate whether receptor functional coupling underlies the protective effect of gabapentin against the alcohol cytotoxic effects.

Item Type: Thesis (Diploma thesis)
Keywords: alcohol, long-term treatment, recombinant GABAA receptors, HEK 293 cell culture; gabapentin
Supervisor: Švob Štrac, Dubravka
Co-supervisor: Đikić, Domagoj and Erhardt, Julija
Date: 2012
Number of Pages: 61
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 04 Mar 2014 13:00
Last Modified: 02 May 2017 09:34
URI: http://digre.pmf.unizg.hr/id/eprint/519

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