The role of molecular chaperone Hsp42 in metabolism regulation of Saccharomyces cerevisiae

Kurtović, Matea (2016) The role of molecular chaperone Hsp42 in metabolism regulation of Saccharomyces cerevisiae. Diploma thesis, Faculty of Science > Department of Chemistry.

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Abstract

The cell actively maintains a balanced relation between protein synthesis, preservation of their stability and conformation, and degradation of damaged proteins. Molecular chaperons are proteins with the important role of maintaining protein homeostasis (proteostasis). In this thesis two yeast strais were newly constructed, hsp42snf1 and ∆hsp42∆zwf1.In both strains a gene for molecular chaperone Hsp42 was deleted, along with the target genes which encode for proteins that participate in glucose metabolism; Snf1 kinase and glucose-6-phosphate dehidrogenase (zwf1). Measurments of the ATP levels, protein carbonylation levels, ROS levels, frequency of mutation and chronological lifespan were performed. The results demonstrate that a mild stress like the chaperone deletion extends the chronological lifespan and induces stress tolerability, while the chronological lifespan is shortened in the strain ∆hsp42∆snf1.It is also shown that frequency of mutation in the above mentioned strains is highly increased.

Item Type: Thesis (Diploma thesis)
Keywords: proteostasis, molecular chaperones, Hsp42, yeast metabolism, lifespan, oxydative stress, Snf1 kinase, reactive oxygen species, ATP
Supervisor: Gruić-Sovulj, Ita
Date: 2016
Number of Pages: 77
Subjects: NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology
Divisions: Faculty of Science > Department of Chemistry
Depositing User: Branka Maravic
Date Deposited: 21 Oct 2016 09:57
Last Modified: 21 Oct 2016 09:57
URI: http://digre.pmf.unizg.hr/id/eprint/5210

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