Dynamics of mitotic spindle assembly

Kuzmić, Barbara (2016) Dynamics of mitotic spindle assembly. Diploma thesis, Faculty of Science > Department of Chemistry.

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Abstract

At the onset of division, the cell forms a spindle, a molecular machine responsible for equal distribution of chromosomes between the daughter cells. It is a highly complex structure made of microtubules and associated proteins. During prometaphase, kinetochore microtubules extend from poles on either end of the cell and attach to the kinetochores, multiprotein structures on the centromeres of the chromosomes. In this thesis, fluorescence microscopy was used for live-cell imaging of human cells. U2OS (CENP-A-GFP, mCherry-α- tubulin) cells were visualized and analyzed. It was shown that the spindle length decreases and the width increases during the prometaphase. Despite the changes in spindle length and width, the contour of this cell structure remains unchanged.

Item Type: Thesis (Diploma thesis)
Keywords: mitosis, prometaphase, mitotic spindle, microtubules, kinetochores, U2OS cells, fluorescence microscopy
Supervisor: Tolić, Iva
Date: 2016
Number of Pages: 47
Subjects: NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology
Divisions: Faculty of Science > Department of Chemistry
Depositing User: Branka Maravic
Date Deposited: 21 Oct 2016 13:08
Last Modified: 21 Oct 2016 13:08
URI: http://digre.pmf.unizg.hr/id/eprint/5213

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