The effect of silver nanoparticles on appearance of oxidative stress and protein expression in tobacco seedlings (Nicotiana tabacum)

Biba, Renata (2016) The effect of silver nanoparticles on appearance of oxidative stress and protein expression in tobacco seedlings (Nicotiana tabacum). Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Silver nanoparticles (AgNP) are excellent antibacterial and antifungal agents found in various consumer products. Due to their potential toxicity numerous studies are being conducted to determine whether they pose a threat to ecosystems and human health. In this research, the effects of AgNP on tobacco seedlings (Nicotiana tabacum) were analysed and compared with the effects of ionic silver (AgNO3) of the same concentrations. AgNP was toxic when applied in higher concentrations, while AgNO3 showed harmful effects even in lower concentrations. The measured increase of reactive oxygen species indicated appearance of oxidative stress, which was proven by the increase in contents of malondialdehyde and protein carbonyls. Both silver forms caused damage to DNA molecules, which was more pronounced in AgNO3 treatments. Antioxidative enzymes showed a change in activity and expression; the activity of SOD and APX increased, while PPX and CAT activity decreased in all treatments with AgNP and AgNO3. Change in protein patterns was also observed, with emphasis on the expression of stress protein Hsp70. Results indicate that AgNO3 is more toxic for plants than AgNP; however, higher AgNP concentrations also induce oxidative stress and affect protein expression, making them potential environmental hazards.

Item Type: Thesis (Diploma thesis)
Keywords: reactive oxygen species, lipid peroxidation, protein carbonyls, DNA damage, antioxidative enzymes, stress proteins
Supervisor: Balen, Biljana and Cvjetko, Petra
Date: 2016
Number of Pages: 64
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 21 Nov 2016 10:30
Last Modified: 21 Nov 2016 10:30
URI: http://digre.pmf.unizg.hr/id/eprint/5311

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