RECOMBINANT GABAA RECEPTORS: IN VITRO MODEL TO STUDY THE EFFECTS OF NEUROSTEROID DEHYDROEPIANDROSTERONE SULPHATE

Tudor, Lucija (2016) RECOMBINANT GABAA RECEPTORS: IN VITRO MODEL TO STUDY THE EFFECTS OF NEUROSTEROID DEHYDROEPIANDROSTERONE SULPHATE. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Dehydroepiandrosterone sulfate (DHEAS) is a neurosteroid involved in various important brain functions such as neural plasticity, learning, memory and behavior, demonstrating the potential to treat a variety of neuropsychiatric disorders, including ischemic stroke. Effects of DHEAS in the central nervous system are mediated through GABAergic, glutamatergic and other neurotransmitter systems. The aim of this study was to investigate the effects of DHEAS on recombinant GABAA receptors by comparing the effects of this neurosteorid on human embryonic kidney (HEK) 293 cells, stably transfected and untransfected with 122S GABAA receptors, and to explore the potential neuroprotective effect of this neurosteroid using in vitro model of oxygen and glucose deprivation and reperfusion (OGDR). The study has shown that DHEAS acts as an allosteric antagonist of recombinant GABAA receptors, but that its prolonged administration does not affect the number, affinity, or the functional coupling of receptor binding sites. The results also suggested that DHEAS, applied both before and after OGDR has protective effect on HEK 293 cells, and that cytoprotective effect of this neurosteroid is at least partly achieved via GABAA receptors.

Item Type: Thesis (Diploma thesis)
Keywords: Dehydroepiandrosterone sulfate (DHEAS); human embryonic kidney (HEK) 293 cells; recombinant GABAA receptors; model of oxygen and glucose deprivation and reperfusion (OGDR); cytoprotective effect; ishemic brain injury
Supervisor: Švob Štrac, Dubravka
Co-supervisor: Benković, Vesna
Date: 2016
Number of Pages: 62
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 23 Nov 2016 10:01
Last Modified: 23 Nov 2016 10:01
URI: http://digre.pmf.unizg.hr/id/eprint/5332

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