Effects of mitochondrial complex I inhibition on the viability of normal human fibroblasts

Jasmin, Božo (2016) Effects of mitochondrial complex I inhibition on the viability of normal human fibroblasts. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

One of the basic characteristics of cells in higher mammals is limited cell division and the ability to enter cellular senescence. Two main mechanisms contribute to cellular senescence. telomere shortening and damaging effects of free radicals. These two mechanisms are in mutual interaction. These data were clearly demonstrated in the experiment in which cell growth and senescence, as well as telomere shortening, showed to be dependent on oxygen partial pressure. The purpose of this research was to determine whether the dynamics of cell growth and the telomere shortening depend on the changes in mitochondrial metabolism or on the action of oxygen itself, under the condition of changed partial pressure of oxygen. Accordingly, we inhibited mitochondrial oxidative system in normal human fibroblasts using rotenone, a complex I inhibitor. We monitored cell growth and senescence under increasing concentrations of rotenone. Cellular senescence was followed by SA-β-Gal reaction and telomere shortening by Southern blot analysis. Mitochondrial mass was measured by flow cytometry and fluorescence microscopy. The results showed changes in mitochondrial metabolism in dependence of rotenon concentrations, as well as changes in cell growth, SA-β-Gal index and telomere shortening dynamics.

Item Type: Thesis (Diploma thesis)
Keywords: rotenone, complex I, reactive oxygen species, SA-β-Gal, mitochondrial mass, telomeres
Supervisor: Rubelj, Ivica
Co-supervisor: Matulić, Maja
Date: 2016
Number of Pages: 67
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 25 Nov 2016 11:06
Last Modified: 25 Nov 2016 11:06
URI: http://digre.pmf.unizg.hr/id/eprint/5343

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