Binding of indole compounds to human albumin

Sekelj, Maja (2016) Binding of indole compounds to human albumin. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Human serum albumin (HSA) is one of the most important transport proteins in human serum. Albumin binds various endogenous and exogenous substances. In pathological conditions such as renal failure and in liver disease, there are present uremic compounds which bind to albumin and thereby inhibit the binding of drugs leading to ineffective therapies. One of the uremic compounds is indole-3-acetic acid (IAA) which has been shown to specifically bind to albumin in pathological conditions. This study investigated the binding of IAA, L- tryptophan, 5-methyl-IAA and 5-benzyloxy-IAA to HSA in the absence and presence of fatty acids, which are normally bound to HSA. By using methods of equilibrium dialysis and fluorescence spectroscopy, the concentrations of unbound and bound ligands were determined, and the data were analyzed according to Scatchard plot. Indole compounds are binding specifically to one binding site of the albumin with different affinities. IAA binds with the highest affinity as compared to other studied indoles.

Item Type: Thesis (Diploma thesis)
Keywords: ligand, protein, fluorescence spectroscopy, stoichiometry of binding, Scatchard plot
Supervisor: Ilakovac Kveder, Marina
Co-supervisor: Peharec Štefanić, Petra
Date: 2016
Number of Pages: 48
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 28 Nov 2016 09:57
Last Modified: 28 Nov 2016 09:57
URI: http://digre.pmf.unizg.hr/id/eprint/5352

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