In vitro modelling of the infuence of extracts from Hermetia illucens larvae on wound healing

Majnarić, Mislav (2016) In vitro modelling of the infuence of extracts from Hermetia illucens larvae on wound healing. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Larvae of black soldier fly, Hermetia illucens, are scavengers who live in extremely unpleasant and harsh environments, such as manure and compost, populated by bacteria and fungi. Carcasses of dead animals and rotting plants could be degraded by larvae of black soldier fly. These biological characteristics suggest that the larvae of black soldier fly are rich in antimicrobial peptides (AMPs) and other substances which possess the activity to fight against resistant bacterial strains. Wound healing is a complex process consisting of four overlapping but strictly defined phases: hemostasis, inflammatory phase, proliferative phase and the remodelling phase (maturation and epithelialization). All phases of wound healing are dependent on the secretion of a variety of cellular compounds such as growth factors, chemokines, cytokines, proteinases, and extracellular matrix proteins. In vitro process of wound healing could be monitored using these different cellular compounds as molecular markers. Focus of this research is enrichment of extracts from H. illucens larvae in order to obtain purified AMPs and other antibiotic compounds and in vitro monitoring of the impact of extracts from H. illucens larvae on bacterial growth and cytotoxicity of human cells. Extracts showed high capacity of inhibition of bacterial growth, especially species Pseudomonas fluorescens. Moreover, majority of extracts which inhibited bacterial growth did not show citotoxic effect on human cells.

Item Type: Thesis (Diploma thesis)
Keywords: Black soldier fly (Hermetia illucens), larval extracts, Hep G2-human liver cancer cell line, Normal human dermal fibroblasts (NHDF), antimicrobial effect, wound healing
Supervisor: Gutzeit, Herwig O.
Co-supervisor: Vojta, Aleksandar
Date: 2016
Number of Pages: 53
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 28 Nov 2016 10:02
Last Modified: 28 Nov 2016 10:02
URI: http://digre.pmf.unizg.hr/id/eprint/5353

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