Adhesion of phytoplankton cells at a charged electrode/aqueous electrolyte interface

Grubeša, Ivana (2016) Adhesion of phytoplankton cells at a charged electrode/aqueous electrolyte interface. Diploma thesis, Faculty of Science > Department of Chemistry.

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Abstract

In this thesis growth and adaptation of marine phytoplankton Dunaliella tertiolecta was examined in two media of different salinity using electrochemical methods and thin layer chromatography. Electrochemical characterization of cell suspensions showed similar adhesion behavior of cells in the wide range of applied potentials at the electrode in the both media. Higher physiological activity of cells due to the presence of exudates of biomolecules and micrometer sized particles was detected in the lower salinity medium. Thin layer chromatography showed increase in concentration of the total lipids with the cell density, for the both media. Higher concentration of total lipids was found in the lower ionic strength. Determined predominant membrane lipids were glycolipids that varied with aging of the cell culture at lower and higher salinity. Combination of electrochemical methods and thin layer chromatography enables better understanding of behavior of phytoplankton cells in aquatic system under environmental stress condition (e.g. variation of salinity).

Item Type: Thesis (Diploma thesis)
Keywords: adhesion, chromatography, chronoamperometry, D. tertiolecta, lipids, phytoplankton
Supervisor: Ivošević DeNadris, Nadica and Močibob, Marko
Date: 2016
Number of Pages: 48
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Faculty of Science > Department of Chemistry
Depositing User: Branka Maravic
Date Deposited: 23 Dec 2016 10:02
Last Modified: 23 Dec 2016 10:02
URI: http://digre.pmf.unizg.hr/id/eprint/5395

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