Role of ganglioside biosynthesis genetic polymorphism and gangliosides expression in T lymphocytes membranes in cervical cancer development

Danolić, Damir (2016) Role of ganglioside biosynthesis genetic polymorphism and gangliosides expression in T lymphocytes membranes in cervical cancer development. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Department of Medical Biology and Genetics, Faculty of Medicine, University of Osijek Cervical cancer is the most common gynaecological cancer in women. Cell mediated immunity plays a significiant role in progression or regression of neoplastic cervical lesions caused by human papilloma virus infection. Engagement of antigen-specific T cell receptors is a prerequisite for T cell activation. The initial events of T cell activation involves movement of the T cell receptor into specialized microdomains known as lipid rafts. Gangliosides play an active role in the formation, stabilization and biological functions of lipid rafts. This study aims to determine whether polymorphisms in the genes involved in the biosynthesis of gangliosides and gangliosides expression in T cell membrane represent risk factor for cervical cancer. Taqman methods for single nucleotide polymorphism genotyping was used. All subjects carried the homozygous wild-type genotypes for all analyzed genes (CC for gene B4GALT5, AA for gene ST3GAL5, AA for gene ST8SIA1 and CC for gene B4GALNT1). Gangliosides expression in T lymphocytes membranes was analyzed using double labelling antibodies on ganglioside GM1. We have not found difference in gangliosides expression in T lymphocytes membranes between the two groups.

Item Type: Thesis (Doctoral thesis)
Keywords: cervical cancer, lipid rafts, cell-mediated immunity, gangliosides, polymorphism
Supervisor: Heffer, Marija and Puljiz, Mario
Date: 2016
Number of Pages: 71
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 11 Jan 2017 11:35
Last Modified: 11 Jan 2017 11:35
URI: http://digre.pmf.unizg.hr/id/eprint/5397

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