Neuroleptic properties of urapidil in condition in vitro and in vivo

Radić, Božo (2016) Neuroleptic properties of urapidil in condition in vitro and in vivo. Doctoral thesis, Faculty of Science > Department of Biology.

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Abstract

Neuroleptic properties of urapidil were investigated in vitro and in vivo models in rats. Urapidil is an antihypertensive agent, agonist of central 5-HT1a and antagonist of peripheral alpha 1 receptors, with the effect on dopaminergic transmission. On PC12 and SH-SY5Y cells urapidil at doses of 100 and 200 µM is not acting like antioxidant (like quercetin does), nor stops cell death (like haloperidol) caused by methamphetamine, but blocks the activity of effector caspase 3/7 mitochondrial pathway. In vivo at a dose of 0.25, 0.5 and 1 mg/rat intraperitoneally (i.p.) it increases locomotion, but without causing abnormal movement or stereotypy like metamphetamine does. At doses of 15, 20 and 25 mg/rat i.p. urapidil causes progressive catalepsy. When applied as pretreatment and posttreatment it antagonizes metamphetamine induced hyperlocomotion and stereotypy. Urapidil blocks the activity of caspase 3/7 following administration of methamphetamine and expresses clear neuroleptic properties likely by binding to dopamine (D2) receptors.

Item Type: Thesis (Doctoral thesis)
Keywords: urapidil, neuroleptics, metamphetamine, cells PH12, cells SYHS5, rats
Supervisor: Sikirić, Predrag and Jazvinšćak Jembrek, Maja
Date: 2016
Number of Pages: 116
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 11 Jan 2017 11:48
Last Modified: 11 Jan 2017 11:48
URI: http://digre.pmf.unizg.hr/id/eprint/5398

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