Genotoxic effect of anesthetic sevoflurane and ionizing radiation in Swiss albino mice

Krsnik, Dajana (2016) Genotoxic effect of anesthetic sevoflurane and ionizing radiation in Swiss albino mice. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Despite of many advantages of various medical aplications (i.e. radiotherapy), radiation can be harmful and it's well known as carcinogen for living organisms. The main biomolecule of interest is DNA. Sevoflurane is nonflammable, inhalation anesthetic which provides fast and efficient hypnosis with low accumulation in tissue and minimal irritation of respiratory tract. However, metabolism of sevoflurane may have an important influence for its toxicity. This research studied individual and combined effect of ionizing radiation (2 Gy) and sevoflurane (2,4 %) on DNA damage in peripheral blood leukocytes as well as in liver, kidney and brain cells of Swiss albino mice. Effect has been observed by alkaline comet assay immediately after, and 2, 6 and 24 hours after exposure to radiation and anesthesia. Results have shown that the most significant genotoxic effect in peripheral blood leukocytes and kidney cells was 2 hours after treatment, whilst in liver and brain cells the cytotoxicity was at the highest level 24 hours after exposure to these agents. Cell repair indeks, used to assess the difference in the amount of DNA repair among various cell types, shown that the least sensitive to treatment with genotoxic agents were peripheral blood leukocytes, and the most sensitive were liver cells.

Item Type: Thesis (Diploma thesis)
Keywords: leukocytes, liver, kidney, brain, comet assay, cellular DNA repair indeks
Supervisor: Benković, Vesna
Date: 2016
Number of Pages: 53
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 19 Jan 2017 13:23
Last Modified: 19 Jan 2017 13:23
URI: http://digre.pmf.unizg.hr/id/eprint/5433

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