Resistance mechanisms to third generation cephalosporins in bacteria Enterobacter spp.

Bartulović, Ana (2012) Resistance mechanisms to third generation cephalosporins in bacteria Enterobacter spp. . Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Strains of Enterobacter spp. are important causes of nosocomial infections. Resistance to antibiotics, especially to third generation cephalosporins, is constantly increasing. Resistance can be mediated through production of inducible β-lactamases, derepressed chromosomal β-lactamases, extended spectrum β-lactamases (ESBL) or through combination of these mechanisms. The purpose of this research was to clarify the resistance mechanisms to antibiotics in question. Disk-diffusion method showed the rise of bacterial resistance to third generation cephalosporins, which was confirmed by MIC method. Cephoxitinecephotaxime antagonistic test showed that none of the isolates produced inducible chromosomal AmpC β-lactamases. Double disk-synergy test showed that all the isolates were ESBL positive, which was confirmed by CLSI test. All the isolates showed chromosomal AmpC β-lactamase hyperproduction, which was detected by boronic acid test. Modified Hodge test showed that two bacterial strains were producing carbapenemases. PCR method showed that 43% of the isolates was positive to TEM β- lactamase production and 60% of the isolates was positive to CTX-M β-lactamase production.

Item Type: Thesis (Diploma thesis)
Keywords: Enterobacter spp., ESBL, AmpC, TEM, CTX-M
Supervisor: Bedenić, Branka and Ivančić Baće, Ivana
Date: 2012
Number of Pages: 48
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Mar 2014 10:13
Last Modified: 18 Sep 2014 12:35
URI: http://digre.pmf.unizg.hr/id/eprint/552

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