Characterization of carbapenemases in Enterobacteriaceae

Barišić, Marina (2012) Characterization of carbapenemases in Enterobacteriaceae. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Carbapenems are antibiotics that are frequently used to treat infections with multidrug-resistant Enterobacteriaceae because they are not inactivated by most β-lactamases. However, β-lactamase mediated resistance to carbapenems has been reported in Enterobacteriaceae, mostly due to the expression of KPC, VIM, NDM or OXA-48 β-lactamase. The purpose of this research was to clarify the resistance mechanisms to carbapenem antibiotics. The isolates were gathered in several hospital institutions in Croatia. The antimicrobial susceptibility to a wide range of antibiotics was determined by disk-diffusion and MIK test. Detection of ESBLs was done by CLSI confirmatory test, detection of metallo-β-lactamases was done by imipenem/meropenem-EDTA test and detection of KPC enzime was done by 3-aminophenylboronic acid test. The presence of genes encoding β-lactamases was determined by PCR using primers for ESBL, OXA-48, KPC, IMP, VIM, NDM i plasmid-mediated AmpC beta-lactamases. The fact that most of the strain possessed not only blacarb genes but also blaESBL and plasmid-mediated blaampC genes limits therapeutic options.

Item Type: Thesis (Diploma thesis)
Keywords: AmpC, ESBLs, KPC, metallo-β-lactamases, multidrug-resistance
Supervisor: Bedenić, Branka
Co-supervisor: Krajačić, Mladen
Date: 2012
Number of Pages: 69
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Mar 2014 10:29
Last Modified: 28 Apr 2017 09:29
URI: http://digre.pmf.unizg.hr/id/eprint/555

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