Cytomegaloviral proteins involved in immunoregulation of mouse poliovirus receptor

Krbavčić, Martina (2012) Cytomegaloviral proteins involved in immunoregulation of mouse poliovirus receptor. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Cytomegalovirus (CMV) is a betaherpesvirus and thus strictly species specific. This prevents the research of human CMV (HCMV) infection in animals. HCMV and murine CMV (MCMV) possess several genes in their genome whose purpose is to negatively regulate immune responses of the host. In line with this, the reduction of surface expression of PVR (poliovirus receptor, CD155), a ligand for immune receptors, was observed on the surface of HCMV infected cells. The aim of this study was to determine MCMV genes that are responsible for the PVR regulation, to produce viral proteins which regulate the expression of PVR molecules and to establish methods for studying the PVR/TIGIT interaction. Biochemical analysis has shown the capacity of viral proteins encoded by m19-m21 region to regulate PVR molecules in MCMV infected mouse fibroblasts. The protein m21 was successfully produced in E. coli expression system. Immune receptor TIGIT that participates in the regulation of immune responses by binding to PVR was successfully isolated from transfected cell lines.

Item Type: Thesis (Diploma thesis)
Keywords: CMV, immune subversion, lymphocytes, PVR, TIGIT
Supervisor: Lenac Roviš, Tihana
Co-supervisor: Oršolić, Nada
Date: 2012
Number of Pages: 72
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 10 Mar 2014 11:59
Last Modified: 10 Mar 2014 11:59
URI: http://digre.pmf.unizg.hr/id/eprint/564

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