Paired arrangement of kinetochores together with microtubule pivoting and dynamics drive kinetochore capture in meiosis I

Cojoc, Gheorghe and Florescu, Ana-Maria and Krull, Alexander and Klemm, Anna H. and Pavin, Nenad and Jülicher, Frank and Tolić, Iva Marija (2016) Paired arrangement of kinetochores together with microtubule pivoting and dynamics drive kinetochore capture in meiosis I. Scientific Reports, 6. 25736-1-25736-11. ISSN 2045-2322

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Abstract

Kinetochores are protein complexes on the chromosomes, whose function as linkers between spindle microtubules and chromosomes is crucial for proper cell division. The mechanisms that facilitate kinetochore capture by microtubules are still unclear. In the present study, we combine experiments and theory to explore the mechanisms of kinetochore capture at the onset of meiosis I in fission yeast. We show that kinetochores on homologous chromosomes move together, microtubules are dynamic and pivot around the spindle pole, and the average capture time is 3-4 minutes. Our theory describes paired kinetochores on homologous chromosomes as a single object, as well as angular movement of microtubules and their dynamics. For the experimentally measured parameters, the model reproduces the measured capture kinetics and shows that the paired configuration of kinetochores accelerates capture, whereas microtubule pivoting and dynamics have a smaller contribution. Kinetochore pairing may be a general feature that increases capture efficiency in meiotic cells.

Item Type: Article
Keywords: kinetochore capture, microtubule pivoting, microtubule dynamics
Date: 11 May 2016
Subjects: NATURAL SCIENCES > Biology
NATURAL SCIENCES > Physics
Additional Information: © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/. Received: 28 January 2016. Accepted: 21 April 2016. Published: 11 May 2016.
Divisions: Faculty of Science > Department of Physics
Publisher: Nature Publishing Group
Depositing User: Gordana Stubičan Ladešić
Date Deposited: 30 Oct 2017 12:12
Last Modified: 30 Oct 2017 12:12
URI: http://digre.pmf.unizg.hr/id/eprint/5641

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