Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore

Cojoc, Gheorghe and Roscioli, Emanuele and Zhang, Lijuan and García-Ulloa, Alfonso and Shah, Jagesh V. and Berns, Michael W. and Pavin, Nenad and Cimini, Daniela and Tolić, Iva Marija and Gregan, Juraj (2016) Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore. Journal of Cell Biology, 212 (7). pp. 767-776. ISSN 0021-9525

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Abstract

Accurate chromosome segregation depends on proper kinetochore– microtubule attachment. Upon microtubule interaction, kinetochores are subjected to forces generated by the microtubules. In this work, we used laser ablation to sever microtubules attached to a merotelic kinetochore, which is laterally stretched by opposing pulling forces exerted by microtubules, and inferred the mechanical response of the kinetochore from its length change. In both mammalian PtK1 cells and in the fission yeast Schizosaccharomyces pombe, kinetochores shortened after microtubule severing. Interestingly, the inner kinetochore–centromere relaxed faster than the outer kinetochore. Whereas in fission yeast all kinetochores relaxed to a similar length, in PtK1 cells the more stretched kinetochores remained more stretched. Simple models suggest that these differences arise because the mechanical structure of the mammalian kinetochore is more complex. Our study establishes merotelic kinetochores as an experimental model for studying the mechanical response of the kinetochore in live cells and reveals a viscoelastic behavior of the kinetochore that is conserved in yeast and mammalian cells.

Item Type: Article
Keywords: kinetochore, chromosome segregation, mitosis, laser ablation, fluorescence microscopy, quantitative modeling
Date: 28 March 2016
Subjects: NATURAL SCIENCES > Biology
NATURAL SCIENCES > Physics
Additional Information: © 2016 Cojoc et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). Submitted: June 2, 2015. Accepted: February 25, 2016. Published March 21, 2016.
Divisions: Faculty of Science > Department of Physics
Project code: RGY0069/2010, MCB- 0842551, MCB-1517506, P21437, P23609, EC (PCIG11-GA-2012-322300), APVV (APVV- 0334-12), VEGA (1/0196/14)
Publisher: Rockefeller University Press
Depositing User: Gordana Stubičan Ladešić
Date Deposited: 30 Oct 2017 13:17
Last Modified: 30 Oct 2017 13:17
URI: http://digre.pmf.unizg.hr/id/eprint/5642

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