Synthesis and application of gold nanoparticles to MDA-MB-231 breast cancer cells

Hanžić , Nikolina (2013) Synthesis and application of gold nanoparticles to MDA-MB-231 breast cancer cells . Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The aim of this work was to study the radiosensitization effect of three types of gold nanoparticles (GNPs) on MDA-MB-231 cancer cells in vitro. GNPs were synthesized in order to be coated with citrate, glutathione and amino-dextrane. Their size was determined using electron microscopy and UV-VIS spectrophotometer and was around 15 nm. GNPs were applied to a MDA-MB-231 cancer cell line and their accumulation in cells was investigated. Transmission electron microscopy demonstrated accumulation of GNPs in cytoplasmic vesicles, while the Mass Spectroscopy was used to demonstrate the highest uptake of amino- dextrane coated GNPs. Cells treated with GNPs were γ-irradiated and their radiosensitization effect was studied. Short-term MTT viability assay showed no cells sensitivity to the effect of ionizing radiation, whereas the long-term clonogenic assay showed high sensitivity to the cytotoxic effect of γ-irradiation. However, the high cytotoxicity of γ-irradiation alone to cancer cells did not allow the determination of the radiosensitization effect of synthesized GNPs.

Item Type: Thesis (Diploma thesis)
Keywords: gold, citrate, glutathione, dextrane, cancer, γ-irradiation, radiosensitization
Supervisor: Gotić, Marijan
Co-supervisor: Marijanović,, Inga and Josipović, Pavle
Date: 2013
Number of Pages: 44
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 11 Mar 2014 17:39
Last Modified: 11 Mar 2014 17:39
URI: http://digre.pmf.unizg.hr/id/eprint/571

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