Mechanisms of resistance to β-lactam antibiotics in gram-negative bacteria

Veir, Zoran (2014) Mechanisms of resistance to β-lactam antibiotics in gram-negative bacteria . Doctoral thesis, Faculty of Science > Department of Biology.

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Gram-negative bacteria are considered to be the most important pathogens causing serious nosocomial infections especially wound infections. The increasing resistance to numerous antibiotics together with other risk factors in hospitalized patients are contributing to development of multiresistant bacteria in recent years. The aim of this study was to determine the mechanisms of antibiotic resistance in: Acinetobacter baumannii, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis and Pseudomonas aeruginosa isolated from wound swabs. ß-lactamases of TEM, SHV and CTX-M family were found in Enterobacteriaceae. Only one isolate of K. pneumoniae produced plasmid-mediated ß-lactamase of CMY family and metallo-β-lactamase of VIM family. All isolates of P. mirabilis harboured blaTEM-52 genes and were clonally related. A. baumannii isolates belonged to a single clone and harboured only blaOXA-51 gene upregulated by ISAba1. The low prevalence of VIM metal-β-lactamase was demonstrated in P. aeruginosa and all of them belonged to the same cluster. Resistance to colistin was not detected in nonfermentative bacteria. The results of this study will give a better insight into molecular epidemiology and enable to establish guidelines for the treatment of these infections.

Item Type: Thesis (Doctoral thesis)
Keywords: gram-negative bacteria, β-lactamases, resistance, antibiotics
Supervisor: Beader, Nataša
Date: 2014
Number of Pages: 99
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 13 Mar 2014 14:01
Last Modified: 23 Sep 2014 10:19

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