Comparison of osteogenic potential of human mesenchymal stem cells isolated from muscle and tendon tissue

Repić, Tihana (2014) Comparison of osteogenic potential of human mesenchymal stem cells isolated from muscle and tendon tissue. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Human mesenchymal stem cells (hMSC) are adult multipotent stem cells that have ability to self-renew and differentiate into several tissues of mesenchymal origin, including bone. It makes them suitable for application in regenerative orthopedics. In this research hMSC were successfully isolated from muscle and tendon tissue which was confirmed by the expression of markers of stemness, Oct4, Sox2 and Nanog. Isolated hMSC were stimulated to osteogenic differentiation after the treatmant with osteoinductive molecules. Both muscle-derived stem cells (MDSC) and tendon-derived stem cells (TDSC) possess osteogenic potential. Differentiated MDSC had higher levels of alkaline phosphatase activity and increased mineralization of bone matrix. Analysis of proteins showed that MDSC expressed higher protein levels of collagen type I, while TDSC expressed higher protein levels of osteocalcin. These results indicate that MDSC might be a better cell source than TDSC for skeletal tissue regeneration.

Item Type: Thesis (Diploma thesis)
Keywords: mesenchymal stem cells, osteogenesis, stemness, regeneration of bone, osteocalcin, collagen type I
Supervisor: Marijanović, Inga
Co-supervisor: Matić, Igor
Date: 2014
Number of Pages: 44
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 19 Mar 2014 08:52
Last Modified: 19 Mar 2014 08:52
URI: http://digre.pmf.unizg.hr/id/eprint/629

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