MInteraction of cholesterol and antioxidant system

Dominko, Kristina (2014) MInteraction of cholesterol and antioxidant system. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Aim of this study was to determine how cholesterol-rich diet influences the cholesterol concentration and oxidative stress levels in mice (C57BL) liver and brain. Also, aim was to determine the levels of antioxidant defense systems in cell model CHOwt and NPC1null (CHO cells with deleted gene for NPC1 protein which accumulate cholesterol) in normal conditions and under higher levels of oxidative stress. In this study the amount of lipid peroxidation, concentration of total glutathione and activity of antioxidant enzymes superoxide dismutase and catalase was measured. Results have shown that the brain is more protected from oxidative stress caused by hypercholesterolemia than the liver. Cells NPC1null have higher oxidative stress levels in normal conditions. Under conditions of higher oxidative stress, cells NPC1null have lower antioxidant defense. In animal and cell models, mitochondria are especially susceptible to oxidative stress caused by cholesterol accumulation and the most affected antioxidant is superoxide dismutase.

Item Type: Thesis (Diploma thesis)
Keywords: cholesterol, oxidative stress, lipid peroxidation, glutathione, superoxide dismutase, catalase, NPC1
Supervisor: Đikić, Domagoj and Katušić Hećimović, Silva
Date: 2014
Number of Pages: 60
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 19 Mar 2014 09:05
Last Modified: 19 Mar 2014 09:05
URI: http://digre.pmf.unizg.hr/id/eprint/631

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