Influence of human proteins α-enolase and MBP-1 on telomere stabilization in mice fibroblasts A9

Božić, Tanja (2014) Influence of human proteins α-enolase and MBP-1 on telomere stabilization in mice fibroblasts A9 . Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Mice fibroblasts A9 have telomeres ~9 kb in length. Introducing the human chromosome 1 or human proteins coded from the same chromosome, α-enolase or MBP-1 into A9 cells, causes telomere lenght reduction to ~4 kb. Change in the equilibrium point to shorter lengths could suggest a change in telomere conformation and their increased stability. For that reason I have verified the telomere length in normal and hybrid fibroblasts A9 and determined the occurrence of t-circles which indicate telomere instability. Telomere length was verified by Southern blot analysis, and t-circles were analysed by 2D gel electrophoresis and by t-circle amplification assay based on rolling circle amplification. Results demonstrate telomere shortening in hybrid A9 cells and significantly lower occurance of t-circles. Introduction of human chromosome 1 or human proteins α-enolase or MBP-1 into A9 cells causes telomere shortening and their stabilization and therefore their telomere dynamics resembles the human.

Item Type: Thesis (Diploma thesis)
Keywords: A9 cells, α-enolase, MBP-1, telomeres, t-circles
Supervisor: Rubelj, Ivica
Date: 2014
Number of Pages: 65
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 19 Mar 2014 09:49
Last Modified: 19 Mar 2014 09:49
URI: http://digre.pmf.unizg.hr/id/eprint/639

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