Seasonal changes in biomarkers of oxidative stress in the amphipod Gammarus fossarum Koch

Kovarik, Ivana (2014) Seasonal changes in biomarkers of oxidative stress in the amphipod Gammarus fossarum Koch. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Due to the anthropogenic influences, freshwater ecosystems are among the most endangered ecosystems in the world and their contamination may affect all other ecosystems. Therefore, it is necessary to assess changes in the environment (biomonitoring), which indicate not only anthropogenic impact but also contribution of natural abiotic and biotic factors on selected markers in water and biota. In this thesis total dissolved metal concentrations in water and biomarkers of oxidative stress (electron transport system activity and lipid peroxidation) in indicator organism, gammaridae Gammarus fossarum, were evaluated. Biomonitornig was performed seasonally (February, April, July, November) at two locations, Sutla River which is the site directly impacted by glass production facility and Črnomerec Stream as a referent station. Increased metal concentrations in Sutla River water indicated higher exposure to metals. Their concentrations were the highest in July and November, while biomarkers of oxidative stress reflected the metabolic changes of gammaride specimens, which are related to ecological factors. Determination of metal distribution among cytosolic proteins confirmed the binding of Cu and Cd with a specific metal binding protein (metallothionein) in the cell cytosol.

Item Type: Thesis (Diploma thesis)
Keywords: biomarkers , metals , oxidative stress , Sutla River , Črnomerec Stream , electron transport activity , malondyaldehide
Supervisor: Filipović Marijić, Vlatka
Co-supervisor: Matoničkin Kepčija, Renata and Sertić Perić, Mirela
Date: 2014
Number of Pages: 66
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 19 Mar 2014 14:09
Last Modified: 19 Mar 2014 14:09
URI: http://digre.pmf.unizg.hr/id/eprint/657

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