SSB protein genes analysis in the genomes of bacteria from the genus 'Candidatus Phytoplasma'

Pinterić, Marija (2014) SSB protein genes analysis in the genomes of bacteria from the genus 'Candidatus Phytoplasma'. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Phytoplasmas (‘Candidatus Phytoplasma’) are bacteria without cell wall that colonize plants and some groups of insects. They have repeat rich genomes with extrachromosomal elements that are mainly composed of multicopy genes. One of those multicopy genes is ssb gene. SSB proteins, essential for cell survival, are found in all domains of life as well as in viruses. In phytoplasma genomes, SSB protein genes may be present in different numbers but there is only one original copy of ssb gene that has different evolutionary origin from the others. Original copy of ssb gene from a number of 'Ca. P. asteris' and 'Ca. P. solani' isolates originated from different areas of Croatia was amplified by PCR and sequenced. Phylogenetic analysis of ssb gene sequences has shown that original copy of ssb gene is conserved. In addition, one copy ssb gene was confirmed in the genomes of ‘Ca. P. asteris’ isolates, whilst there is another copy present in the plant isolates of ‘Ca. P. solani’ that isn’t present in genome isolate of ‘Ca. P. solani’ obtained from insect vector.

Item Type: Thesis (Diploma thesis)
Keywords: phytoplasma, ssb gene, original copy, phylogenetic analysis, Southern blot
Supervisor: Šeruga Musić, Martina
Date: 2014
Number of Pages: 70
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 19 Mar 2014 14:24
Last Modified: 19 Mar 2014 14:24
URI: http://digre.pmf.unizg.hr/id/eprint/660

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