Effects of guanylin peptids on bradykinin signaling pathway in HEK293 cell culture

Rabik, Karolina (2013) Effects of guanylin peptids on bradykinin signaling pathway in HEK293 cell culture. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The occlusion of cerebral arteries leads to a stroke and consequently to a cerebral edema, which contributes to an increase of ischemic injury. In this study we investigate the effect of guanylin peptides, guanylin and uroguanylin and guanylate cyclase C agonist on the signaling system of bradykinin, which is the main contributor to the occurrence of cerebral edema after the stroke. The aim of this study is to clarify the activity mechanism of the guanylin peptides on HEK293 cells and their effect on bradykinin, as we have recently shown on the example of guanylate cyclase agonist A, atrial natriuretic peptide (ANP) and urodilatin. In this experiment we used slow-whole cell patch-clamp technique to measure a membrane potential in HEK293 cells. We showed that guanylin and uroguanylin, just like their secondary messenger cyclic GMP, depolarize a plasma membrane and they inhibit the activity of bradykinin signaling pathway via an activation of RGS protein. Results of this study suggest a possible protective role of guanylin peptides in a decrease of cerebral edema and also a decrease of an ischemic brain injury.

Item Type: Thesis (Diploma thesis)
Keywords: natriuretic peptides, guanylin, uroguanylin, patch-clamp
Supervisor: Sinđić, Aleksandra
Date: 2013
Number of Pages: 37
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 25 Mar 2014 12:02
Last Modified: 27 Apr 2017 12:51
URI: http://digre.pmf.unizg.hr/id/eprint/701

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