Effects of salinity and osmotic stress on protein differential expression in Mammillaria gracilis Pfeiff. in vitro-grown cultures

Rogić, Tea (2014) Effects of salinity and osmotic stress on protein differential expression in Mammillaria gracilis Pfeiff. in vitro-grown cultures. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Although cacti Mammillaria gracilis Pfeiff. is not commercially interesting plant species in Europe, its adaptations on arid environments make it suitable model organism for studying biochemical and molecular mechanisms which activate during exposure of plants to salt and osmotic stress. Cacti tissues grow in vitro as habituated calli of unorganised growth capable of regeneration potential retention, and as completely unorganised tumors induced via transformation of cacti cells with Agrobacterium tumefaciens bacteria. This research showed that calli and tumor tissues respond to salt and osmotic stress at the level of soluble cell proteins synthesis. Mechanisms underlying these responses differ and are dependent of tissue used, but it seems that tumor tissue is, at least to some extent, more sensitive to impact of stress factors than calli. Differential expression protein analysis implicated heat shock protein family, as well as glyceraldehyde-3-phosphate dehydrogenase protein, as general markers of salt and osmotic stress presence in plants.

Item Type: Thesis (Diploma thesis)
Keywords: cactus, salt stress, osmotic stress, 2D electrophoresis, mass spectrometry
Supervisor: Balen, Biljana and Cindrić, Mario
Date: 2014
Number of Pages: 86
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 25 Mar 2014 13:36
Last Modified: 23 Sep 2014 10:03
URI: http://digre.pmf.unizg.hr/id/eprint/707

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