Genome stability of zebrafish (Danio rerio Hamilton 1822) after exposure to model genotoxicants

Lazić, Aleksandar (2014) Genome stability of zebrafish (Danio rerio Hamilton 1822) after exposure to model genotoxicants. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

DNA damage as a consequence of toxicant exposure may in the long run influence health and survival of the organisms. Therefore, it is of paramount importance to develop and test sensitive molecular cytogenetic methods for early detection and assessment of these changes. The aim of this study was to assess DNA damage in zebrafish (Danio rerio) exposed to model genotoxicants (Benzo[a]pyrene (B[a]P), Ethyl methanesulfonate (EMS)) and to determine the longevity of such damage. The primary DNA damage was detected using Comet assay whereas the long-term effects were monitored using the AFLP (amplified fragment length polymorphism) method. The results of this study demonstrated the primary DNA damage caused by model genotoxicants as well as persistence of alterations in the zebrafish genome even one year after the end of the genotoxic exposure. Zebrafish was confirmed as suitable model organism in studies of genotoxicity.

Item Type: Thesis (Diploma thesis)
Keywords: genotoxicity, EMS, B[a]P, comet assay, AFLP
Supervisor: Klobučar, Goran
Date: 2014
Number of Pages: 34
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 26 Mar 2014 13:03
Last Modified: 16 Feb 2016 14:01
URI: http://digre.pmf.unizg.hr/id/eprint/716

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