Complex formation between protein p63 and mutant p53 in human tumor cell line

Pfeiffer, Anamarija (2014) Complex formation between protein p63 and mutant p53 in human tumor cell line. Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

The tumor suppressor gene TP53 is mutated in over 50% of reported human tumor cases. The outcomes of the mutation are either loss of p53 function or gain of function resulting in oncogenic phenotype. Some of the mutant p53 oncogenic features are explained through interactions with other p53 family members, specifically tumor suppressor isoforms TAp63 and TAp73. A common polymorphism on codon 72 affects the strength of these interactions. Expression vectors coding for TAp63α or mutant p53 with different codon 72 polymorphism were cotransfected into H1299 cells, derived from human lung carcinoma metastasis, which have a partial deletion of the TP53 gene. Protein complexes were isolated from cell lysates using the co-immunoprecipitation method, and analysed by western blotting. Complexes have been found between TAp63α and p53 mutants L194F and R282W, but not between TAp63α and R175H, R280K and I332A. It was established that mut p53 72R binds more efficiently to TAp63α.

Item Type: Thesis (Diploma thesis)
Keywords: p53 family, protein complexes, tumor suppressors, p53 codon 72 polymorphism
Supervisor: Slade, Neda
Date: 2014
Number of Pages: 55
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 26 Mar 2014 13:54
Last Modified: 26 Mar 2014 13:54
URI: http://digre.pmf.unizg.hr/id/eprint/722

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