The effect of cadmium and zinc and their mixtures on genome stability in tobacco seedlings (Nicotiana tabacum L.)

Bučan, Ana (2014) The effect of cadmium and zinc and their mixtures on genome stability in tobacco seedlings (Nicotiana tabacum L.). Diploma thesis, Faculty of Science > Department of Biology.

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Abstract

Metals are natural components of the Earth’s crust but their concentration is constantly increasing due to anthropologic influences. Heavy metals can cause direct and indirect damages of vital biological molecules and their accumulation in the food chain is an immediate menace for all organisms in the environment. The objective of this research was to determine the effect of heavy metals, cadmium and zinc and their mixtures on the genome stability of tobacco seedlings. Even though the influences of these metals alone in plant organisms are researched in great detail, their interaction is not well known. Since zinc and cadmium are chemically very similar heavy metals, cadmium can interfere in vital, biological reactions inside the plants by replacing zinc. Seedlings were grown for 30 days on nutrient medium with addition of different metal concentrations and their mixtures. The extent of DNA damage and DNA alterations after the treatment were evaluated using the Comet assay and RAPD (Random Amplified Polymorphic DNA) method. Even though the results of Comet assay showed statistically significant DNA damage caused by heavy metals, individually and in mixtures, the results of RAPD analysis did not show any change in DNA polymorphism in all of the treatments. Comet assay proved to be a more sensitive method in detection of DNA damages caused by heavy metals in comparison to RAPD analysis.

Item Type: Thesis (Diploma thesis)
Keywords: heavy metals , genotoxicity , comet assay , DNA polymorphism , cadmium , zinc , tobacco
Supervisor: Pavlica, Mirjana
Date: 2014
Number of Pages: 38
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 02 Apr 2014 10:06
Last Modified: 02 Apr 2014 10:06
URI: http://digre.pmf.unizg.hr/id/eprint/759

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