Animal models of psoriasis: anti-inflammatory and antioxidant epigallocatehin gallate efficiency

Balta, Vedran (2014) Animal models of psoriasis: anti-inflammatory and antioxidant epigallocatehin gallate efficiency. Diploma thesis, Faculty of Science > Department of Biology.

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Psoriasis is a chronic, immune-mediated disease, which immunopathogenesis, prevention and treatments are not completely resolved. We did some researches about positive effects of epigallocatehin gallate (EGCG) preparations on an animal model of psoriasis, in which the psoriasis was caused by di-n-propyl disulfide (PPD) irritant during 5 days. Anti-inflammatory and anti-oxidative activity of ECGC preparations were followed by various methods: valuation of cell numbers in abdominal cavity, functional activity of macrophages in peritoneal fluid, pathohistological analyses of transformations on experimental animals, hematological and biochemical parameters of blood and method of valuation of anti-oxidative efficiency (reduced glutathione, lipid peroxidation). The results indicate that EGCG preparations reduce number of inflammatory cells in peritoneal fluid of mice. All hematological and biochemical transformations indicate that it falls within the reference range and the results of cytokines' values in plasma indicate that PPD alone, or in aggregation with EGCG, caused a bigger level of anti-inflammatory cytokines, especially colony stimulating factore hormones (G-CSF) and interleukins-6 (IL-6). Pathohistological analysis of skin samples indicates that a topical use of the irritant with tested substances, in all groups, causes significantly lower inflammatory reaction than PPD itself. We estimated genotoxic effect of examined preparations with the comet test and the micronucleus test. The results of tests show no genotoxicity induced by ECGC preparations in peripheral blood cells of mice. We concluded that researched ECGC preparations showed anti-inflammatory effect. It could be used for suppression and treatment of inflammatory presents in psoriasis, without any toxical effects.

Item Type: Thesis (Diploma thesis)
Keywords: flavonoids, psoriasis, thermography, oxidative stress, inflammation
Supervisor: Oršolić, Nada
Date: 2014
Number of Pages: 51
Subjects: NATURAL SCIENCES > Biology
Divisions: Faculty of Science > Department of Biology
Depositing User: Grozdana Sirotic
Date Deposited: 08 Apr 2014 12:44
Last Modified: 23 Sep 2014 08:24

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