High Resolution Structure of the Manganese Derivative of Insulin

Prugovečki, Biserka and Pulić, Ivana and Toth, Martina and Matković-Čalogović, Dubravka (2012) High Resolution Structure of the Manganese Derivative of Insulin. Croatica Chemica Acta, 85 (4). pp. 435-439. ISSN 0011-1643

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Abstract

The structure of the human manganese derivative of insulin was determined using the single crystal X-ray diffraction method. Single crystals were grown by the hanging drop vapour diffusion crystallization method using zinc-free insulin and manganese(II) sulphate monohydrate in citrate buffer at pH = 6.4. It crystallizes in the trigonal system in the space group R3 with the unit cell parameters: a = b = 81.70 Å and c = 33.73 Å. There are two manganese ions per insulin hexamer in the human manganese-insulin derivative. The manganese ions lie on the three-fold crystallographic axis on opposite sides of the hexamer. Both manganese ions are octahedrally coordinated by three Nε2 atoms from the imidazolyl side chains of three symmetry-related HisB10/HisD10 and three oxygen atoms of three symmetry-related water molecules. The investigated human manganese-insulin derivative adopts the T6 conformation.

Item Type: Article
Keywords: insulin derivative; manganese; X-ray structure
Date: 2012
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Faculty of Science > Department of Chemistry
Publisher: Hrvatsko kemijsko društvo
Related URLs:
Depositing User: Branka Maravic
Date Deposited: 28 Apr 2014 12:44
Last Modified: 28 Apr 2014 12:44
URI: http://digre.pmf.unizg.hr/id/eprint/990

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